Journal: bioRxiv
Article Title: The spatial landscape of infectious mononucleosis tonsils
doi: 10.1101/2025.10.09.681345
Figure Lengend Snippet: a. Schematic overview of the multiomic spatial single-cell workflow. b. OPAL mIF staining of developed and validated EBV-panel showing co-expression of EBV viral latent antigens EBNA1, EBNA2, LMP1 & LMP2A as well as lytic protein BZLF1. c. PhenoCycler-FUSION mIF staining of full-face IM tonsil tissue showing human CD20 (red), pan-cytokeratin (yellow), CD3e (blue), CD68 (green) and the EBV viral protein LMP1 (purple). d. Uniform manifold approximation and projection (UMAP) of all 51,895 cells captured by CosMx spatial transcriptomics (ST), coloured and labelled by the 9 populations identified from Louvain single-cell clustering. e. Dot plot showing the average gene expression of the top differentially expressed genes per cluster, canonical lineage markers as well as EBV genes for the CosMx ST annotated populations. f. UMAP of re-clustered epithelial cells from CosMx ST showing columnar (basal), polygonal (intermediate) and squamous (superficial) phenotypes. g. Differential gene expression analysis of EBV+ vs. EBV-epithelial cells highlighting EBV EBERs and IgG expression h. Representative images of EBV protein expression in the PF mIF data showing co-expression with pan-cytokeratin.
Article Snippet: & G.S.T. acquired the CosMx spatial transcriptomics data; C.I.L.
Techniques: Staining, Expressing, Gene Expression